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KMID : 0369820080380050295
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Jorunal of Korean Pharmaceutical Sciences
2008 Volume.38 No. 5 p.295 ~ p.302
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The Regulation of Taurine Transport through the Blood-Placental Barrier under Oxidative Stress
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Kang Young-Sook
Yoon Yoo-Geum
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Abstract
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In the present study, we examined the changes of uptake and efflux of taurine under various conditions inducing oxidative stress using rat conditionally immortalized syncytiotrophoblast cell line, TR-TBT cell, as blood-placental barrier in vitro model. In addition, we identified the characteristics of taurine transport in TR-TBT cells including general features, besides effect of calcium ion on taurine transport. Taurine uptake showed time, Na+ and Cl- dependency, and was decreased by PKC activator in TR-TBT cells. Also, calcium free condition decreased taurine uptake and evoked taurine efflux in the cells. Oxidative stress induced the change of taurine transport in TR-TBT cells, but the changes were different depending on the types of stimulation inducing oxidative stress. The taurine uptake was increased by TNF-α, LPS and DEM stimulation but decreased by H2O2 and NO stimulation. Also, the taurine efflux was regulated by TNF-α stimulation. In conclusion, the taurine transport through the blood-placental barrier was regulated in oxidative stress conditions, and these results demonstrated that oxidative stress affected the taurine supplies to fetus and taurine level of fetus.
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KEYWORD
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Blood-placental barrier, TR-TBT, Taurine transporter, Oxidative stress, Calcium ion
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